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assay kit  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology assay kit
    Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tbars+colorimetric+assay+kit/Thiobarbituric+Acid+Reactants+(TBARS)+Colorimetric+Assay+Kit/pm41962334-57-2-4
    Average 94 stars, based on 38 article reviews
    assay kit - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Colorimetric Assay:

    Article Title: Metformin hydrochloride improves hepatic glucolipid metabolism in diabetes progression through SIRT5-mediated ECHA desuccinylation
    Article Snippet: The blood glucose was measured using a glucometer (Roche, Mannheim, Germany) after injection of 0, 15, 30, 60, 90 and 120 min. .. The levels of thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), adenosine triphosphate (ATP) and H 2 O 2 in liver tissues of mice, as well as glucose consumption of HepG2 cells were detected using a TBARS colorimetric assay kit (Elabscience, Wuhan, China), a SOD assay kit (Beyotime), an ATP assay kit (Beyotime), a H 2 O 2 assay kit (Beyotime) and a glucose assay kit (Beyotime), respectively. ..

    Article Title: Inhibitory effects of citral on the production of virulence factors in Staphylococcus aureus and its potential application in M preservation.
    Article Snippet: Foodborne diseases caused by Staphylococcus aureus contamination on meat and meat products has gained increasing attention in recent years, while the pathogenicity of S. aureus is mainly attributed to its virulence factors production, which is primarily regulated by quorum sensing (QS) system.. Herein, we aimed to uncover the inhibitory effects and mechanisms of citral (CIT) on virulence factors production by S. aureus, and further explore its potential application in pork preservation.. Susceptibility test confirmed the antibacterial properties of CIT against S. aureus, the minimal inhibitory concentration (MIC) was 0.25 mg/mL.

    Article Title: Passively-targeted mitochondrial tungsten-based nanodots for efficient acute kidney injury treatment
    Article Snippet: CCK-8 and SOD assay kit were purchased from Dojindo Molecular Technologies (Kumamoto, Japan). .. Mouse KIM-1, HO-1, TNF-α and IL-6 ELISA kits and TBARS Colorimetric Assay kit were purchased from Elabscience Biotechnology (Houston, Texas, USA). .. A TUNEL assay kit (C10617), Caspase 3 polyclonal antibody (PA577887), Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed secondary antibody Alexa Fluor 488 (A11034), Goat anti-Rabbit IgG (H + L) Cross-Adsorbed secondary antibody Alexa Fluor 555 (A21428), MitoSOXTM Red Mitochondrial Superoxide Indicator (M36008) and ProLongTM Glass Antifade Mountant with NucBlueTM Stain (P36983) were obtained from Thermo Fisher Scientific ( Carlsbad, CA, USA).

    Article Title: Knockdown of EFEMP1 Promotes Ferroptosis by Inactivating PI3K/AKT to overcome the Resistance of Hepatocellular Carcinoma Cells to Sorafenib.
    Article Snippet: .. Thiobarbituric Acid Reactive Substances Assay Thiobarbituric Acid Reactive Substances (TBARS) level in HCCLM3, HCCLM3-SR, Huh7 and Huh7-SR cells was detected with a TBARS Colorimetric Assay kit (E-BC-K298-M, Elabscience, Wuhan, China). ..

    Article Title: Fabrication of alginate dialdehyde-gelatin crosslinked hydrogels incorporated with cinnamaldehyde nanoparticles for meat preservation.
    Article Snippet: Meat spoilage caused by Salmonella Typhimurium (S. typhimurium) contamination has become a major concern worldwide.. Herein, the antibacterial mechanisms of cinnamaldehyde (CIN) against S. typhimurium were evaluated, and the alginate dialdehyde (ADA)-gelatin (Gel) crosslinked hydrogels loaded with CIN nanoparticles prepared by zein and carboxymethyl chitosan (ZCC/CINNPs) were fabricated for meat preservation.. Results showed the efficient antibacterial effects of CIN via interfering with the S. typhimurium cell membrane, increasing the membrane permeability, disrupting the membrane integrity, thereby leading to the intracellular materials leakage and cell death.

    ATP Assay:

    Article Title: Metformin hydrochloride improves hepatic glucolipid metabolism in diabetes progression through SIRT5-mediated ECHA desuccinylation
    Article Snippet: The blood glucose was measured using a glucometer (Roche, Mannheim, Germany) after injection of 0, 15, 30, 60, 90 and 120 min. .. The levels of thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), adenosine triphosphate (ATP) and H 2 O 2 in liver tissues of mice, as well as glucose consumption of HepG2 cells were detected using a TBARS colorimetric assay kit (Elabscience, Wuhan, China), a SOD assay kit (Beyotime), an ATP assay kit (Beyotime), a H 2 O 2 assay kit (Beyotime) and a glucose assay kit (Beyotime), respectively. ..

    Glucose Assay:

    Article Title: Metformin hydrochloride improves hepatic glucolipid metabolism in diabetes progression through SIRT5-mediated ECHA desuccinylation
    Article Snippet: The blood glucose was measured using a glucometer (Roche, Mannheim, Germany) after injection of 0, 15, 30, 60, 90 and 120 min. .. The levels of thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), adenosine triphosphate (ATP) and H 2 O 2 in liver tissues of mice, as well as glucose consumption of HepG2 cells were detected using a TBARS colorimetric assay kit (Elabscience, Wuhan, China), a SOD assay kit (Beyotime), an ATP assay kit (Beyotime), a H 2 O 2 assay kit (Beyotime) and a glucose assay kit (Beyotime), respectively. ..

    other:

    Article Title: Metformin hydrochloride improves hepatic glucolipid metabolism in diabetes progression through SIRT5-mediated ECHA desuccinylation.
    Article Snippet: The blood glucose was measured using a glucometer (Roche, Mannheim, Germany) after injection of 0, 15, 30, 60, 90 and 120 min.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Passively-targeted mitochondrial tungsten-based nanodots for efficient acute kidney injury treatment
    Article Snippet: CCK-8 and SOD assay kit were purchased from Dojindo Molecular Technologies (Kumamoto, Japan). .. Mouse KIM-1, HO-1, TNF-α and IL-6 ELISA kits and TBARS Colorimetric Assay kit were purchased from Elabscience Biotechnology (Houston, Texas, USA). .. A TUNEL assay kit (C10617), Caspase 3 polyclonal antibody (PA577887), Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed secondary antibody Alexa Fluor 488 (A11034), Goat anti-Rabbit IgG (H + L) Cross-Adsorbed secondary antibody Alexa Fluor 555 (A21428), MitoSOXTM Red Mitochondrial Superoxide Indicator (M36008) and ProLongTM Glass Antifade Mountant with NucBlueTM Stain (P36983) were obtained from Thermo Fisher Scientific ( Carlsbad, CA, USA).

    Serial Dilution:

    Article Title: Fabrication of alginate dialdehyde-gelatin crosslinked hydrogels incorporated with cinnamaldehyde nanoparticles for meat preservation.
    Article Snippet: Meat spoilage caused by Salmonella Typhimurium (S. typhimurium) contamination has become a major concern worldwide.. Herein, the antibacterial mechanisms of cinnamaldehyde (CIN) against S. typhimurium were evaluated, and the alginate dialdehyde (ADA)-gelatin (Gel) crosslinked hydrogels loaded with CIN nanoparticles prepared by zein and carboxymethyl chitosan (ZCC/CINNPs) were fabricated for meat preservation.. Results showed the efficient antibacterial effects of CIN via interfering with the S. typhimurium cell membrane, increasing the membrane permeability, disrupting the membrane integrity, thereby leading to the intracellular materials leakage and cell death.



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    Effects of PAQR3 overexpression on ferroptosis in HCC cells. (a) <t>Lipid</t> <t>peroxidation</t> levels measured by <t>TBARS</t> assay. (b, c) ROS levels detected by C11-BODIPY staining (red/green ratio; scale bars: 50 μm). (d, e) Intracellular Fe 2+ levels assessed by fluorescence intensity (scale bars: 50 μm). (f) Representative western blot bands of ferroptosis-related proteins (SLC7A11, GPX4, ACSL4, and b-actin). (g) Quantification of relative SLC7A11 expression. (h) Quantification of relative GPX4 expression. (i) Quantification of relative ACSL4 expression. ✶ ✶ ✶ P < 0.001, ns: Not significant. Data in (a, g, h, i) are presented as mean±standard deviation from three independent experiments. Data in (b-e) and blot in (f) are representative of three independent experiments. PAQR3: Progestin and adipoQ receptor 3, HCC: Hepatocellular carcinoma, TBARS: Thiobarbituric acid reactive substance, ROS: Reactive oxygen species, Oe-NC: Overexpression negative control.
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    Effects of PAQR3 overexpression on ferroptosis in HCC cells. (a) <t>Lipid</t> <t>peroxidation</t> levels measured by <t>TBARS</t> assay. (b, c) ROS levels detected by C11-BODIPY staining (red/green ratio; scale bars: 50 μm). (d, e) Intracellular Fe 2+ levels assessed by fluorescence intensity (scale bars: 50 μm). (f) Representative western blot bands of ferroptosis-related proteins (SLC7A11, GPX4, ACSL4, and b-actin). (g) Quantification of relative SLC7A11 expression. (h) Quantification of relative GPX4 expression. (i) Quantification of relative ACSL4 expression. ✶ ✶ ✶ P < 0.001, ns: Not significant. Data in (a, g, h, i) are presented as mean±standard deviation from three independent experiments. Data in (b-e) and blot in (f) are representative of three independent experiments. PAQR3: Progestin and adipoQ receptor 3, HCC: Hepatocellular carcinoma, TBARS: Thiobarbituric acid reactive substance, ROS: Reactive oxygen species, Oe-NC: Overexpression negative control.
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    Effects of PAQR3 overexpression on ferroptosis in HCC cells. (a) <t>Lipid</t> <t>peroxidation</t> levels measured by <t>TBARS</t> assay. (b, c) ROS levels detected by C11-BODIPY staining (red/green ratio; scale bars: 50 μm). (d, e) Intracellular Fe 2+ levels assessed by fluorescence intensity (scale bars: 50 μm). (f) Representative western blot bands of ferroptosis-related proteins (SLC7A11, GPX4, ACSL4, and b-actin). (g) Quantification of relative SLC7A11 expression. (h) Quantification of relative GPX4 expression. (i) Quantification of relative ACSL4 expression. ✶ ✶ ✶ P < 0.001, ns: Not significant. Data in (a, g, h, i) are presented as mean±standard deviation from three independent experiments. Data in (b-e) and blot in (f) are representative of three independent experiments. PAQR3: Progestin and adipoQ receptor 3, HCC: Hepatocellular carcinoma, TBARS: Thiobarbituric acid reactive substance, ROS: Reactive oxygen species, Oe-NC: Overexpression negative control.
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    Effects of PAQR3 overexpression on ferroptosis in HCC cells. (a) <t>Lipid</t> <t>peroxidation</t> levels measured by <t>TBARS</t> assay. (b, c) ROS levels detected by C11-BODIPY staining (red/green ratio; scale bars: 50 μm). (d, e) Intracellular Fe 2+ levels assessed by fluorescence intensity (scale bars: 50 μm). (f) Representative western blot bands of ferroptosis-related proteins (SLC7A11, GPX4, ACSL4, and b-actin). (g) Quantification of relative SLC7A11 expression. (h) Quantification of relative GPX4 expression. (i) Quantification of relative ACSL4 expression. ✶ ✶ ✶ P < 0.001, ns: Not significant. Data in (a, g, h, i) are presented as mean±standard deviation from three independent experiments. Data in (b-e) and blot in (f) are representative of three independent experiments. PAQR3: Progestin and adipoQ receptor 3, HCC: Hepatocellular carcinoma, TBARS: Thiobarbituric acid reactive substance, ROS: Reactive oxygen species, Oe-NC: Overexpression negative control.
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    Image Search Results


    Effects of PAQR3 overexpression on ferroptosis in HCC cells. (a) Lipid peroxidation levels measured by TBARS assay. (b, c) ROS levels detected by C11-BODIPY staining (red/green ratio; scale bars: 50 μm). (d, e) Intracellular Fe 2+ levels assessed by fluorescence intensity (scale bars: 50 μm). (f) Representative western blot bands of ferroptosis-related proteins (SLC7A11, GPX4, ACSL4, and b-actin). (g) Quantification of relative SLC7A11 expression. (h) Quantification of relative GPX4 expression. (i) Quantification of relative ACSL4 expression. ✶ ✶ ✶ P < 0.001, ns: Not significant. Data in (a, g, h, i) are presented as mean±standard deviation from three independent experiments. Data in (b-e) and blot in (f) are representative of three independent experiments. PAQR3: Progestin and adipoQ receptor 3, HCC: Hepatocellular carcinoma, TBARS: Thiobarbituric acid reactive substance, ROS: Reactive oxygen species, Oe-NC: Overexpression negative control.

    Journal: CytoJournal

    Article Title: A novel candidate tumor biomarker progestin and adipoQ receptor 3 regulates cell metastasis through transforming growth factor-β pathway in hepatocellular carcinoma

    doi: 10.25259/Cytojournal_138_2025

    Figure Lengend Snippet: Effects of PAQR3 overexpression on ferroptosis in HCC cells. (a) Lipid peroxidation levels measured by TBARS assay. (b, c) ROS levels detected by C11-BODIPY staining (red/green ratio; scale bars: 50 μm). (d, e) Intracellular Fe 2+ levels assessed by fluorescence intensity (scale bars: 50 μm). (f) Representative western blot bands of ferroptosis-related proteins (SLC7A11, GPX4, ACSL4, and b-actin). (g) Quantification of relative SLC7A11 expression. (h) Quantification of relative GPX4 expression. (i) Quantification of relative ACSL4 expression. ✶ ✶ ✶ P < 0.001, ns: Not significant. Data in (a, g, h, i) are presented as mean±standard deviation from three independent experiments. Data in (b-e) and blot in (f) are representative of three independent experiments. PAQR3: Progestin and adipoQ receptor 3, HCC: Hepatocellular carcinoma, TBARS: Thiobarbituric acid reactive substance, ROS: Reactive oxygen species, Oe-NC: Overexpression negative control.

    Article Snippet: Lipid peroxidation in HCC cells following the indicated treatments was assessed using a TBARS Assay Kit (Elabscience Biotechnology Co., Ltd., E-BC-K298-M, Wuhan, Hubei, China).

    Techniques: Over Expression, TBARS Assay, Staining, Fluorescence, Western Blot, Expressing, Standard Deviation, Negative Control

    PAQR3 regulates HCC cell metastasis and ferroptosis through the TGF-b pathway. (a) Wound healing assay images showing cell migration in different groups (scale bars: 200 μm). (b) Quantification of cell migration rates from wound healing assay. (c) Transwell invasion assay images of HCC cells in different groups (scale bars: 50 μm). (d) Quantification of invaded cell counts from the Transwell assay. (e) Representative Western blot images of EMT-related proteins (E-cadherin, N-cadherin, Vimentin, b-actin). (f) Quantification of relative E-cadherin expression. (g) Quantification of relative N-cadherin expression. (h) Quantification of relative Vimentin expression. (i) TBARS assay showing lipid peroxidation levels. (j) C11-BODIPY staining showing ROS levels (merge, oxidized C11, non-oxidized C11, scale bars: 50 μm). (k) Representative images of intracellular Fe 2+ fluorescence (scale bars: 50 μm). (l) Quantification of red/green fluorescence ratio for ROS levels. (m) Quantification of intracellular Fe 2+ fluorescence intensity. (n) Representative western blot images of ferroptosis-related proteins (SLC7A11, GPX4, ACSL4, b-actin). (o) Quantification of relative SLC7A11 expression. (p) Quantification of relative GPX4 expression. (q) Quantification of relative ACSL4 expression. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001, ns: Not significant. Images in (a, c, e, j, k, n) are representative of three independent experiments. Quantitative data in (b, d, f-h, i, l, m, o-q) are presented as mean±SD from three independent experiments. PAQR3: Progestin and adipoQ receptor 3, HCC: Hepatocellular carcinoma, EMT: Epithelial-mesenchymal transition, TBARS: Thiobarbituric acid reactive substance, Oe-NC: Overexpression negative control.

    Journal: CytoJournal

    Article Title: A novel candidate tumor biomarker progestin and adipoQ receptor 3 regulates cell metastasis through transforming growth factor-β pathway in hepatocellular carcinoma

    doi: 10.25259/Cytojournal_138_2025

    Figure Lengend Snippet: PAQR3 regulates HCC cell metastasis and ferroptosis through the TGF-b pathway. (a) Wound healing assay images showing cell migration in different groups (scale bars: 200 μm). (b) Quantification of cell migration rates from wound healing assay. (c) Transwell invasion assay images of HCC cells in different groups (scale bars: 50 μm). (d) Quantification of invaded cell counts from the Transwell assay. (e) Representative Western blot images of EMT-related proteins (E-cadherin, N-cadherin, Vimentin, b-actin). (f) Quantification of relative E-cadherin expression. (g) Quantification of relative N-cadherin expression. (h) Quantification of relative Vimentin expression. (i) TBARS assay showing lipid peroxidation levels. (j) C11-BODIPY staining showing ROS levels (merge, oxidized C11, non-oxidized C11, scale bars: 50 μm). (k) Representative images of intracellular Fe 2+ fluorescence (scale bars: 50 μm). (l) Quantification of red/green fluorescence ratio for ROS levels. (m) Quantification of intracellular Fe 2+ fluorescence intensity. (n) Representative western blot images of ferroptosis-related proteins (SLC7A11, GPX4, ACSL4, b-actin). (o) Quantification of relative SLC7A11 expression. (p) Quantification of relative GPX4 expression. (q) Quantification of relative ACSL4 expression. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001, ns: Not significant. Images in (a, c, e, j, k, n) are representative of three independent experiments. Quantitative data in (b, d, f-h, i, l, m, o-q) are presented as mean±SD from three independent experiments. PAQR3: Progestin and adipoQ receptor 3, HCC: Hepatocellular carcinoma, EMT: Epithelial-mesenchymal transition, TBARS: Thiobarbituric acid reactive substance, Oe-NC: Overexpression negative control.

    Article Snippet: Lipid peroxidation in HCC cells following the indicated treatments was assessed using a TBARS Assay Kit (Elabscience Biotechnology Co., Ltd., E-BC-K298-M, Wuhan, Hubei, China).

    Techniques: Wound Healing Assay, Migration, Transwell Invasion Assay, Transwell Assay, Western Blot, Expressing, TBARS Assay, Staining, Fluorescence, Over Expression, Negative Control